Database of genetic profiles at the Department of Forensic Medicine, Medical University of Bialystok.

PURPOSE

The objective of this project was to establish an 'in-house' DNA database to store and compare profiles genotyped in the Department of Forensic Medicine, Medical University of Bialystok.

MATERIAL AND METHODS

DNA was extracted using Chelex-100, an organic procedure, or commercial kits. Genetic profiles were obtained using AmpFISTR SGM Plus, AmpFISTR Profiler, or AmpFISTR Identifiler and 310 ABI Prism Genetic Analyzer (Applera). DNA Stat v. 1. 2 software was used to construct the database.

RESULTS

As of the end of 2006 our forensic database stored 1,595 profiles genotyped in criminal cases in the years 2000-2006, including 398 non-match samples, 2 non-match fetuses, 5 non-match newborns and 4 non-match corpses.

CONCLUSION

A DNA database was established that may be used for the purpose of genetic profile comparison in criminal cases.