Takahashi Hajime, Kimura Bon, Tanaka Yuichiro, Mori Mayumi, Yokoi Asami, Fujii Tateo
Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato, Tokyo 108-8477, Japan.
J Food Prot. 2008 Apr;71(4):839-44. doi: 10.4315/0362-028x-71.4.839.
The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA sequence. As 97.8% of the isolated strains from various foods were grouped correctly, use of the PCR-SSCP method enables the prompt and labor-saving analysis of microbial population of food-derived bacterial strains. Advantages in speed and accuracy of bacterial population identification by the PCR-SSCP method have practical application for food suppliers and testing laboratories.
开发了一种在对16S rDNA的一部分进行PCR扩增后，利用单链构象多态性（SSCP）对食品中分离菌株进行分组的方法。该方法能够根据16S rDNA序列对来自各种食品样品的菌株进行分组。由于来自各种食品的分离菌株中有97.8%被正确分组，因此使用PCR-SSCP方法能够快速且省力地分析食品来源细菌菌株的微生物群落。PCR-SSCP方法在细菌群落鉴定的速度和准确性方面的优势对食品供应商和检测实验室具有实际应用价值。
