Tsubota S I, Huong D V
Department of Biology, University of Michigan, Ann Arbor 48109.
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):693-7. doi: 10.1073/pnas.88.3.693.
A 6.1-kilobase insertion into the rudimentary (r) gene was cloned and partially sequenced. The insertion consists of a 703-base-pair (bp) P element next to a 5.4-kilobase single-copy sequence. The normal position of the single-copy sequence is near the tip of the X chromosome. Upon insertion into the r gene, this chimeric element generated an 8-bp target-site duplication, characteristic of P elements. At the non-P-element end of the insertion, the first 8 bp are identical to the first 8 bp of the inverted terminal repeats of the P element. Thus, this element has inverted terminal repeats of 8 bp. This large element can excise from the r gene under conditions of hybrid dysgenesis, which indicates that it behaves like a normal P element. These data support the conclusion that a normally stable single-copy sequence has now become unstable and duplicated within the genome.
一个插入到残翅（r）基因中的6.1千碱基片段被克隆并进行了部分测序。该插入片段由一个703碱基对（bp）的P因子和一个5.4千碱基的单拷贝序列组成。单拷贝序列的正常位置靠近X染色体的末端。插入到r基因后，这个嵌合元件产生了一个8 bp的靶位点重复序列，这是P因子的特征。在插入片段的非P因子末端，前8 bp与P因子反向末端重复序列的前8 bp相同。因此，这个元件有8 bp的反向末端重复序列。在杂种不育的条件下，这个大元件可以从r基因中切除，这表明它的行为类似于正常的P因子。这些数据支持了这样一个结论：一个通常稳定的单拷贝序列现在在基因组内变得不稳定并发生了重复。
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