Genome. 1995 Aug;38(4):823-6. doi: 10.1139/g95-106.
The random amplified polymorphic DNA technique was used to find markers for a downy mildew resistance gene in sorghum. Of the 674 random primers screened for polymorphism, 2 amplified fragments were linked to a downy mildew resistance gene in sorghum line SC414. Utilization of an existing restriction fragment length polymorphism mapping population (IS3620C x BTx623) also revealed two markers that are linked to a different resistance gene in another sorghum line, BTx623.
随机扩增多态性 DNA 技术被用于在高粱中寻找抗霜霉病基因的标记。在筛选出的 674 个随机引物中,有 2 个扩增片段与高粱品系 SC414 中的一个抗霜霉病基因连锁。利用现有的限制片段长度多态性作图群体(IS3620C x BTx623),还揭示了两个与另一个高粱品系 BTx623 中的不同抗性基因连锁的标记。