Rheumatology Unit Monash Medical Centre Locked Bag No. 29 Clayton 3168 Australia.
Mediators Inflamm. 1993;2(1):49-52. doi: 10.1155/S0962935193000067.
The annexin lipocortin 1 is reported to mediate some anti-inflammatory effects of glucocorticoids, but the mechanisms of this mediation are incompletely understood. The involvement of lipocortin 1 in glucocorticoid inhibition of monocyte interleukin 1beta (IL-1beta) release has been investigated. Treatment of peripheral blood monocytes with 2 mug/ml lipopolysaccharide potently increased IL-1beta release (p = 0.001) and dexamethasone (10(-7) M) significantly reduced both resting and stimulated IL-1beta release (p = 0.009). A neutralizing monoclonal antibody to lipocortin 1 (0.5-50.0 mug/ml) was unable to inhibit this effect and recombinant lipocortin 1 (2 x 10(-6) M) and 188aa lipocortin 1 fragment (10(-8)-10(-6) M) had no effect. It is concluded that lipocortin 1 is not involved in the inhibition of monocyte IL-1beta release by glucocorticoids.
膜联蛋白脂皮质蛋白 1 据报道介导了糖皮质激素的一些抗炎作用,但这种介导的机制尚不完全清楚。已经研究了脂皮质蛋白 1 在糖皮质激素抑制单核细胞白细胞介素 1β(IL-1β)释放中的作用。用 2 μg/ml 脂多糖处理外周血单核细胞可强烈增加 IL-1β释放(p=0.001),并且地塞米松(10(-7)M)显着降低了静止和刺激的 IL-1β释放(p=0.009)。针对脂皮质蛋白 1 的中和单克隆抗体(0.5-50.0 μg/ml)不能抑制这种作用,重组脂皮质蛋白 1(2×10(-6)M)和 188aa 脂皮质蛋白 1 片段(10(-8)-10(-6)M)也没有作用。因此,结论是脂皮质蛋白 1不参与糖皮质激素抑制单核细胞 IL-1β释放。