Zhang Xiao-ying, Wang Xia, Sun Shan-zhen, Song Ying, Yang Mei-xiang, Qu Xun
Department of Oral Pathology, School of Stomatology, Shandong University, Jinan 250012, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Dec;42(12):747-9.
To investigate the effect of hypoxia inducible factor-1 alpha (HIF-1 alpha) on vascular endothelial growth factor (VEGF) expression in Tca8113 cells under hypoxia.
The expression of the mRNA of HIF-1 alpha and VEGF in Tca8113 cells was examined by RT-PCR technique at different culture times (1/2 h, 1 h, 3 h, 6 h, 12 h, 24 h) under normoxic and hypoxic conditions.
The expression of HIF-1 alpha under hypoxia showed the trend of increasing first and then decreasing, and was higher than that of the control (normoxic group) at 6h and 12 h (P < 0.05). The expression of VEGF under hypoxia was higher than that of the control group at 1/2 h, 1 h, 3 h, 12 h, 24 h (P < 0.05). The expression of hypoxia-induced VEGF mRNA increased with the increased expression of HIF-1 alpha mRNA in the cell lines tested at the initial stage of hypoxia. But no statistical significant association was observed between HIF-1 alpha and VEGF expression within 24 h under hypoxia (rs = 0.5750, P > .005).
The increased expression of VEGF in Tca8113 cells might be mediated by multiple factors, including HIF-1 alpha.
研究缺氧诱导因子-1α（HIF-1α）对缺氧条件下Tca8113细胞中血管内皮生长因子（VEGF）表达的影响。
采用RT-PCR技术检测常氧和缺氧条件下不同培养时间（1/2 h、1 h、3 h、6 h、12 h、24 h）Tca8113细胞中HIF-1α和VEGF的mRNA表达。
缺氧条件下HIF-1α的表达呈先升高后降低的趋势，在6 h和12 h时高于对照组（常氧组）（P < 0.05）。缺氧条件下VEGF在1/2 h、1 h、3 h、12 h、24 h时的表达高于对照组（P < 0.05）。在缺氧初期，所检测细胞系中缺氧诱导的VEGF mRNA表达随HIF-1α mRNA表达的增加而增加。但在缺氧24 h内，未观察到HIF-1α与VEGF表达之间有统计学意义的相关性（rs = 0.5750，P >.005）。
Tca8113细胞中VEGF表达增加可能由多种因素介导，包括HIF-1α。
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