Chen Si-Yuan, Takeuchi Satoshi, Moroi Yoichi, Hayashida Sayaka, Kido Makiko, Uchi Hiroshi, Takahara Masakazu, Uenotsuchi Takeshi, Tu Ya-Ting, Urabe Kazunori, Furue Masutaka
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Int J Dermatol. 2008 Jun;47(6):562-6. doi: 10.1111/j.1365-4632.2008.03643.x.
Specificity protein 1 (Sp1) is a transcription factor and shown to be a sequence-specific DNA-binding protein that activate a broad and diverse spectrum of mammalian gene, such as vascular endothelial growth factor (VEGF) gene. But the expression of Sp1 and VEGF has not previously been investigated in extramammary Paget's disease (EMPD).
To investigate the expression of Sp1 and VEGF proteins in EMPD and to assess their relationships and potential contribution to malignant transduction of EMPD, paraffin-embedded EMPD specimens (35 tissue samples from 33 patients with primary EMPD, including two samples of metastatic lymph nodes from two patients) were subjected to immunohistochemical staining for Sp1 and VEGF.
All of the 35 EMPD specimens, including all of six invasive EMPD and two metastatic lymph node specimens, showed strong nuclear positive staining for Sp1 and strong cytoplasmic positive staining for VEGF. The expression levels (% positive cells) of Sp1 and VEGF in EMPD were significantly higher than those of normal skin (NS). There was a significantly high correlation between expression levels of Sp1 and VEGF in EMPD.
The present study reveals that the concordant over-expression of Sp1 and VEGF may play a pivotal role in the tumorigenesis and further malignant transduction of EMPD.
特异性蛋白1（Sp1）是一种转录因子，被证明是一种序列特异性DNA结合蛋白，可激活多种哺乳动物基因，如血管内皮生长因子（VEGF）基因。但此前尚未在乳腺外佩吉特病（EMPD）中研究Sp1和VEGF的表达情况。
为了研究Sp1和VEGF蛋白在EMPD中的表达情况，并评估它们之间的关系以及对EMPD恶性转化的潜在作用，对石蜡包埋的EMPD标本（来自33例原发性EMPD患者的35个组织样本，包括2例患者的2个转移性淋巴结样本）进行Sp1和VEGF的免疫组织化学染色。
35个EMPD标本，包括所有6个浸润性EMPD标本和2个转移性淋巴结标本，均显示Sp1强核阳性染色和VEGF强细胞质阳性染色。EMPD中Sp1和VEGF的表达水平（阳性细胞百分比）显著高于正常皮肤（NS）。EMPD中Sp1和VEGF的表达水平之间存在显著的高度相关性。
本研究表明，Sp1和VEGF的协同过表达可能在EMPD的肿瘤发生和进一步的恶性转化中起关键作用。
