猪脉络丛中的血清素（5-HT1c）受体。

Serotonin (5-HT1c) receptors in pig choroid plexus.

作者信息

Yiin K T, Chen C F, Kuo J S, Tsou C T, Wang J J, Song Y M, Lin S H, Tseng L N, Wang Y S, Lin W H

机构信息

Department of Internal Medicine, Veterans General Hospital-Taichung, Taiwan, R.O.C.

出版信息

Zhonghua Yi Xue Za Zhi (Taipei). 1991 Mar;47(3):149-53.

PMID:1848141

Abstract

It is very important to find suitable reaction conditions to attain a high specific binding (specific/total binding) in the receptor binding study. Membrane homogenates of pig choroid plexus are known to have exclusively serotonin (5-hydroxytryptamine, 5-HT) receptor of the subtype 5-HT1c. In this study, we used the membrane preparation of pig choroid plexus tissue and the specific binding of [3H]5-HT was 72-84% to serotonin receptor subtype 5-HT1c, as defined by the inhibition of 1 uM 5-HT, when a radioligand concentration of 0.5 nM of [3H]5-HT was used in the assay. Analysis of the properties of specific [3H]5-HT binding in pig choroid plexus tissue membrane preparation revealed linear Scatchard plots. In Tris-HCl buffer without CaCl2, pargyline or ascorbic acid, high average of affinity dissociation constant (Kd) of 1.3 +/- 0.2 nM (SEM, n = 4) and also a high average of receptor density (Bmax) of 284 +/- 12 fmol/mg of protein were found. Pig choroid plexus proves to be a good material for 5-HT1c receptor binding study.

摘要

在受体结合研究中，找到合适的反应条件以获得高特异性结合（特异性/总结合）非常重要。已知猪脉络丛的膜匀浆仅具有5-羟色胺（5-羟色胺，5-HT）受体亚型5-HT1c。在本研究中，我们使用了猪脉络丛组织的膜制剂，当在测定中使用0.5 nM的[3H]5-HT放射性配体浓度时，[3H]5-HT与5-HT受体亚型5-HT1c的特异性结合为72-84%，这是由1 μM 5-HT的抑制作用所定义的。对猪脉络丛组织膜制剂中特异性[3H]5-HT结合特性的分析显示出线性Scatchard图。在不含CaCl2、帕吉林或抗坏血酸的Tris-HCl缓冲液中，发现平均亲和力解离常数（Kd）较高，为1.3±0.2 nM（SEM，n = 4），并且受体密度（Bmax）的平均值也较高，为284±12 fmol/mg蛋白质。猪脉络丛被证明是进行5-HT1c受体结合研究的良好材料。