Zhou L, Jones S C P, Angen Ø, Bossé J T, Nash J H E, Frey J, Zhou R, Chen H C, Kroll J S, Rycroft A N, Langford P R
Department of Paediatrics, Imperial College London, St Mary's Campus, London w2 1pg.
Vet Rec. 2008 May 17;162(20):648-52. doi: 10.1136/vr.162.20.648.
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific pcr, based on the capsule locus, which can be used in a multiplex format with the organism's specific gene apxIV. The pcr test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.
猪胸膜肺炎放线杆菌是猪胸膜肺炎的病原体，据报道，其血清型3和8在英国占主导地位。该生物体分离株的直接血清分型通常通过兔血清对其表面多糖的免疫反应性来确定，但该方法存在局限性，例如血清型3、6和8之间的交叉反应。本研究描述了一种基于荚膜基因座的血清型3特异性PCR的开发，该PCR可与该生物体的特异性基因apxIV以多重形式使用。对266株胸膜肺炎放线杆菌和121株其他生物体（包括猪的所有主要呼吸道细菌病原体）进行了PCR检测评估。该检测具有高度特异性和敏感性，对于区分血清型3、6和8的菌株以及在血清型3流行的地区（如英国）进行血清流行率和流行病学调查应该是有用的。
