Vervuert I, Voigt K, Hollands T, Cuddeford D, Coenen M
Institute of Animal Nutrition, Nutritional Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kührstrasse 8, D-04159 Leipzig, Germany.
Vet Rec. 2008 May 24;162(21):684-8. doi: 10.1136/vr.162.21.684.
Four horses were randomly fed a diet containing rolled, micronised or extruded barley; the barley intake was adjusted to supply 2 g starch/kg bodyweight per day. During a 10-day acclimatisation period the horses were also fed 1 kg grass hay/100 kg bodyweight per day. Samples of blood and breath were collected at the end of each period after the test meal of barley had been fed after a 12-hour overnight fast. The glycaemic and insulinaemic responses of the horses were measured as an indication of the pre-caecal digestibility of starch, and postprandial breath hydrogen and methane were measured to detect microbial fermentation of starch. The highest peak serum glucose and serum insulin concentrations were observed after feeding the extruded barley, lower concentrations were observed after feeding the micronised barley and the lowest concentrations were observed after feeding the rolled barley. Breath hydrogen increased within four hours of feeding all the barley diets, and the mean (sd) peak hydrogen concentrations were 98.3 (55.2) ppm for rolled barley, 59.3 (31.5) ppm for micronised barley and 96.1 (51.9) ppm for extruded barley. There were wide variations within individual horses but these concentrations were not significantly different. Breath methane concentrations were very variable and, although there were no significant differences, there was a trend for higher methane concentrations after the feeding of rolled barley.
四匹马被随机喂食包含碾压大麦、微粉化大麦或挤压大麦的日粮；大麦摄入量经调整后为每天每千克体重提供2克淀粉。在为期10天的适应期内，马匹还按每天每100千克体重1千克禾本科干草的量进行投喂。在禁食12小时后投喂大麦测试餐后，于每个阶段结束时采集血液和呼气样本。测定马匹的血糖和胰岛素反应，以指示淀粉在盲肠前的消化率，并测定餐后呼气中的氢气和甲烷，以检测淀粉微生物发酵情况。投喂挤压大麦后观察到最高的血清葡萄糖峰值和血清胰岛素浓度，投喂微粉化大麦后浓度较低，投喂碾压大麦后浓度最低。投喂所有大麦日粮后，呼气氢气在4小时内增加，碾压大麦的平均（标准差）氢气峰值浓度为98.3（55.）ppm，微粉化大麦为59.3（31.5）ppm，挤压大麦为96.1（51.9）ppm。个体马匹之间存在很大差异，但这些浓度并无显著差异。呼气甲烷浓度变化很大，虽然没有显著差异，但投喂碾压大麦后甲烷浓度有升高趋势。
