Han EunHee, Bae Won C, Hsieh-Bonassera Nancy D, Wong Van W, Schumacher Barbara L, Görtz Simon, Masuda Koichi, Bugbee William D, Sah Robert L
Department of Bioengineering, University of California-San Diego, 9500 Gilman Drive, MC 0412, La Jolla, CA 92093-0412, USA.
Clin Orthop Relat Res. 2008 Aug;466(8):1912-20. doi: 10.1007/s11999-008-0291-7. Epub 2008 May 28.
One goal of treatment for large articular cartilage defects is to restore the anatomic contour of the joint with tissue having a structure similar to native cartilage. Shaped and stratified cartilaginous tissue may be fabricated into a suitable graft to achieve such restoration. We asked if scaffold-free cartilaginous constructs, anatomically shaped and targeting spherically-shaped hips, can be created using a molding technique and if biomimetic stratification of the shaped constructs can be achieved with appropriate superficial and middle/deep zone chondrocyte subpopulations. The shaped, scaffold-free constructs were formed from the alginate-released bovine calf chondrocytes with shaping on one (saucer), two (cup), or neither (disk) surfaces. The saucer and cup constructs had shapes distinguishable quantitatively (radius of curvature of 5.5 +/- 0.1 mm for saucer and 2.8 +/- 0.1 mm for cup) and had no adverse effects on the glycosaminoglycan and collagen contents and their distribution in the constructs as assessed by biochemical assays and histology, respectively. Biomimetic stratification of chondrocyte subpopulations in saucer- and cup-shaped constructs was confirmed and quantified using fluorescence microscopy and image analysis. This shaping method, combined with biomimetic stratification, has the potential to create anatomically contoured large cartilaginous constructs.
治疗大型关节软骨缺损的一个目标是用结构类似于天然软骨的组织恢复关节的解剖轮廓。可以将成型且分层的软骨组织制作成合适的移植物来实现这种恢复。我们探讨了是否可以使用成型技术创建无支架软骨构建体，使其具有符合解剖学形状并针对球形髋关节，以及是否可以通过适当的表层和中/深层软骨细胞亚群实现成型构建体的仿生分层。成型的无支架构建体由藻酸盐释放的牛犊软骨细胞形成，在一个（碟形）、两个（杯形）或无（盘形）表面进行成型。碟形和杯形构建体的形状在数量上可区分（碟形的曲率半径为5.5±0.1毫米，杯形为2.8±0.1毫米），并且通过生化分析和组织学评估，对构建体中糖胺聚糖和胶原蛋白的含量及其分布均无不良影响。使用荧光显微镜和图像分析对碟形和杯形构建体中的软骨细胞亚群进行了仿生分层的确认和量化。这种成型方法与仿生分层相结合，有可能创建符合解剖学轮廓的大型软骨构建体。
