重组亚基作为细粒棘球绦虫抗原B结构和功能特性研究的工具
Recombinant subunits as tools for the structural and functional characterization of Echinococcus granulosus antigen B.
作者信息
Monteiro Karina M, Zaha Arnaldo, Ferreira Henrique B
机构信息
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Cx. Postal 15005, 91501-970 Porto Alegre, RS, Brazil.
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Cx. Postal 15005, 91501-970 Porto Alegre, RS, Brazil; Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
出版信息
Exp Parasitol. 2008 Aug;119(4):490-498. doi: 10.1016/j.exppara.2008.04.015. Epub 2008 Apr 24.
Antigen B (AgB) is a major protein component of the Echinococcus granulosus metacestode. It is oligomeric and this raises several questions regarding the subunit structure and composition of AgB. Several genes that encode different AgB subunits have been identified, and some of these have been cloned and expressed to produce recombinant subunits. The study of these recombinant subunits may provide new insights into the structure, physical-chemical properties, and functional aspects of AgB. Like native AgB, the AgB8/1, AgB8/2, and AgB8/3 recombinant subunits produced in our laboratory form 120-160 kDa oligomers that have stable secondary structures, are strongly antigenic and immunogenic, and selectively bind hydrophobic compounds. Here, we review these results and discuss their implications for the elucidation of the structure and function of AgB. This includes a possible role for AgB in host-parasite interactions.
抗原B（AgB）是细粒棘球绦虫中绦期的一种主要蛋白质成分。它是寡聚体，这就引发了几个关于AgB亚基结构和组成的问题。已鉴定出几个编码不同AgB亚基的基因，其中一些已被克隆和表达以产生重组亚基。对这些重组亚基的研究可能为AgB的结构、物理化学性质和功能方面提供新的见解。与天然AgB一样，我们实验室生产的AgB8/1、AgB8/2和AgB8/3重组亚基形成120 - 160 kDa的寡聚体，这些寡聚体具有稳定的二级结构，具有强抗原性和免疫原性，并能选择性地结合疏水性化合物。在此，我们综述这些结果，并讨论它们对阐明AgB结构和功能的意义。这包括AgB在宿主 - 寄生虫相互作用中可能发挥的作用。
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