[健咽特号（JYTH）急性给药的抗抑郁作用及其可能机制]

[Antidepressant effect of acute administration with Jianyate Hao (JYTH) and its possible mechanism].

作者信息

Lv Ping, Li Shan, Wang Ren-Ye, Chen Liang, Ku Bao-Shan, Pan Jian-Chun

机构信息

School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Feb;33(3):307-10.

PMID:18536473

Abstract

OBJECTIVE

Jianyate Hao (JYTH) , a traditional Chinese medicine formula, which has been used effectively to treat depression in the past ten years. The purpose of this study was to explore the antidepressant effect of acute administration with JYTH and its possible mechanism.

METHOD

the animals behavioral despair models of depression, the tail suspension and forced swimming tests, were used to explore the antidepressant effects of JYTH. In addition, the locomotor activity test was used to detect the change of locomotor activity. The monoamine oxidase activity in the mouse brain was also determined by using fluorospectrophotometry.

RESULT

JYTH (17.5, 35, 70 g x kg(-1), ig) could decrease the duration of immobility in both tail suspension and forced swimming tests, and the effect of JYTH (35 g x kg(-1) ig) was resembling imipramine (10 mg x kg(-1), ip) in relieving depression. And the effective doses (17.5, 35, 70 g x kg(-1), ig) did not alter locomotion activity. Moreover, JYTH (35 g x kg(-1), ig) was found to inhibit monoamine oxidase activity in the mouse brain.

CONCLUSION

These findings suggest that JYTH exerts antidepressant effect in animals behavioral despair tests and the underlying mechamism may involve the inhibition monoamine oxidase activity in the mouse brain.

摘要

目的

解郁泰浩（JYTH）是一种中药配方，在过去十年中已被有效用于治疗抑郁症。本研究的目的是探讨急性给予JYTH的抗抑郁作用及其可能的机制。

方法

采用动物行为绝望抑郁模型，即悬尾试验和强迫游泳试验，来探讨JYTH的抗抑郁作用。此外，采用自主活动试验检测自主活动的变化。还通过荧光分光光度法测定小鼠脑中的单胺氧化酶活性。

结果

JYTH（17.5、35、70 g·kg⁻¹，灌胃）可缩短悬尾试验和强迫游泳试验中的不动时间，且JYTH（35 g·kg⁻¹，灌胃）在缓解抑郁方面的效果与丙咪嗪（10 mg·kg⁻¹，腹腔注射）相似。并且有效剂量（17.5、35、70 g·kg⁻¹，灌胃）并未改变自主活动。此外，发现JYTH（35 g·kg⁻¹，灌胃）可抑制小鼠脑中的单胺氧化酶活性。