明胶/间苯二酚/醛作为止血剂和组织粘合剂的评估：大鼠实验研究

Evaluation of gelatin/resorcinol/aldehyde as a hemostatic agent and tissue adhesive: an experimental study in rat.

作者信息

Singh Rajinder P, Maheshwari Veena, Verma Akshay K

机构信息

Transplant and General Surgery, Renal and Transplant Unit, Nottingham University Hospitals, Nottingham, United Kingdom.

出版信息

Int Surg. 2008 Jan-Feb;93(1):25-31.

PMID:18543551

Abstract

Use of gelatin/resorcinol/formaldehyde glue as a tissue adhesive and hemostatic agent was evaluated experimentally after modification of the aldehyde component. Gelatin/ resorcinol/aldehyde (GRA) glue was prepared by mixing gelatin and resorcinol and cross-linking with a blend of formaldehyde/glutaraldehyde. After wedge resection of the liver of 28 albino rats, bleeding was controlled, and the cut surfaces were joined with GRA. Liver biopsy was done at 7, 14, 21, and 28-day intervals. Hemostasis was excellent in 71.4%, satisfactory in 25.0%, and poor in 3.6%. Mean time for tissue adhesion was 2.6 minutes. No necrosis or bile leakage was seen. Visibility of site of repair decreased from 50.0% on day 7 to 14.3% on day 28. Residual glue was seen microscopically on day 28 in 43% cases. GRA is safe and good hemostatic, tissue adhesive, and sealant in rat liver.

摘要

在对醛类成分进行改良后，对明胶/间苯二酚/甲醛胶作为一种组织粘合剂和止血剂的用途进行了实验评估。明胶/间苯二酚/醛（GRA）胶是通过将明胶和间苯二酚混合并用甲醛/戊二醛混合物交联制备而成。对28只白化大鼠的肝脏进行楔形切除后，控制出血，并使用GRA胶连接切面。在第7、14、21和28天间隔进行肝脏活检。止血效果极佳的占71.4%，满意的占25.0%，差的占3.6%。组织粘连的平均时间为2.6分钟。未见坏死或胆汁渗漏。修复部位的可见度从第7天的50.0%降至第28天的14.3%。在第28天，43%的病例在显微镜下可见残留胶水。GRA胶在大鼠肝脏中是安全且良好的止血剂、组织粘合剂和密封剂。