Hickman Zachary A, Swindell Annika L, Allan Ian J, Rhodes Angela H, Hare Rina, Semple Kirk T, Reid Brian J
School of Environmental Sciences, UEA, Norwich NR94QR, UK.
Environ Pollut. 2008 Dec;156(3):1041-5. doi: 10.1016/j.envpol.2008.04.022. Epub 2008 Jun 12.
This study sought to extend validation of a cyclodextrin based extraction method for the assessment of PAH-biodegradation potential to complex multi-contaminant matrices. To this end, four reference materials (RMs) were produced by blending, in different proportions, soils impacted with diesel, lubricating oil and spent oxide. These reference materials had modest summation operatorPAH (16 US EPA) concentrations that ranged from 5.6+/-0.5 to 44.4+/-4.5 mg kg(-1). However, extractable petroleum hydrocarbon (EPH) concentrations were comparatively high (up to 2520+/-204 mg kg(-1)). To complement these RMs, two further soils from a municipal gas plant (MGP) with highly elevated concentration of PAHs ranging from 877+/-52 to 2620+/-344 mg kg(-1) were also tested. Results showed, regardless of matrix complexity, that PAH biodegradation within the four RM substrates, and two MGP soils correlated well with biodegradation predicted by hydroxypropyl-beta-cyclodextrin (HPCD) extraction.
本研究旨在扩展一种基于环糊精的萃取方法的验证范围，该方法用于评估多环芳烃（PAH）在复杂多污染物基质中的生物降解潜力。为此，通过将受柴油、润滑油和废氧化物污染的土壤按不同比例混合，制备了四种参考物质（RM）。这些参考物质中多环芳烃（16种美国环保署规定的物质）的浓度适中，范围为5.6±0.5至44.4±4.5毫克/千克。然而，可萃取石油烃（EPH）的浓度相对较高（高达2520±204毫克/千克）。为补充这些参考物质，还测试了另外两种来自城市煤气厂（MGP）的土壤，其多环芳烃浓度极高，范围为877±52至2620±344毫克/千克。结果表明，无论基质的复杂性如何，四种参考物质基质以及两种城市煤气厂土壤中的多环芳烃生物降解与羟丙基-β-环糊精（HPCD）萃取预测的生物降解具有良好的相关性。
