反射式色度计测量和红外光谱法在杏(Prunus armeniaca L.)类胡萝卜素含量快速无损评估中的应用。

Application of reflectance colorimeter measurements and infrared spectroscopy methods to rapid and nondestructive evaluation of carotenoids content in apricot (Prunus armeniaca L.).

作者信息

Ruiz David, Reich Maryse, Bureau Sylvie, Renard Catherine M G C, Audergon Jean-Marc

机构信息

Unité de Génétique et d'Amélioration des Fruits et Légumes, INRA-Avignon, Domaine St. Maurice, BP-94, 84143 Montfavet, France.

出版信息

J Agric Food Chem. 2008 Jul 9;56(13):4916-22. doi: 10.1021/jf7036032. Epub 2008 Jun 17.

Abstract

The importance of carotenoid content in apricot (Prunus armeniaca L.) is recognized not only because of the color that they impart but also because of their protective activity against human diseases. Current methods to assess carotenoid content are time-consuming, expensive, and destructive. In this work, the application of rapid and nondestructive methods such as colorimeter measurements and infrared spectroscopy has been evaluated for carotenoid determination in apricot. Forty apricot genotypes covering a wide range of peel and flesh colors have been analyzed. Color measurements on the skin and flesh ( L*, a*, b*, hue, chroma, and a*/ b* ratio) as well as Fourier transform near-infrared spectroscopy (FT-NIR) on intact fruits and Fourier transform mid-infrared spectroscopy (FT-MIR) on ground flesh were correlated with the carotenoid content measured by high-performance liquid chromatography. A high variability in color values and carotenoid content was observed. Partial least squares regression analyses between beta-carotene content and provitamin A activity and color measurements showed a high fit in peel, flesh, and edible apricot portion (R(2) ranged from 0.81 to 0.91) and low prediction error. Regression equations were developed for predicting carotenoid content by using color values, which appeared as a simple, rapid, reliable, and nondestructive method. However, FT-NIR and FT-MIR models showed very low R(2) values and very high prediction errors for carotenoid content.

摘要

杏(Prunus armeniaca L.)中类胡萝卜素含量的重要性不仅在于它们赋予果实的颜色,还在于其对人类疾病的保护作用。目前评估类胡萝卜素含量的方法既耗时、昂贵又具有破坏性。在这项研究中,已对诸如色度计测量和红外光谱等快速无损方法在杏类胡萝卜素测定中的应用进行了评估。分析了40种涵盖广泛果皮和果肉颜色的杏基因型。对果实表皮和果肉的颜色测量(L*、a*、b*、色调、色度和a*/b*比值)以及对完整果实的傅里叶变换近红外光谱(FT-NIR)和对磨碎果肉的傅里叶变换中红外光谱(FT-MIR)与通过高效液相色谱法测定的类胡萝卜素含量进行了相关性分析。观察到颜色值和类胡萝卜素含量存在很大差异。β-胡萝卜素含量与维生素A原活性和颜色测量之间的偏最小二乘回归分析表明,在果皮、果肉和可食用杏部分具有高度拟合度(R²范围为0.81至0.91)且预测误差较低。通过使用颜色值建立了预测类胡萝卜素含量的回归方程,这是一种简单、快速、可靠且无损的方法。然而,FT-NIR和FT-MIR模型对类胡萝卜素含量的R²值非常低且预测误差非常高。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索