Hopkins D J, Betenbaugh M J, Dhurjati P
Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA.
Biotechnol Bioeng. 1987 Jan;29(1):85-91. doi: 10.1002/bit.260290113.
The effect of dissolved oxygen shock on the stability of recombinant Escherichia coli cells containing plasmid pKN401 was investigated. The recombinant cells were stable in control batch experiments in media with and without ampicillin. However, these recombinant cells were highly unstable under conditions where a dissolved oxygen shock was induced. The results have implications for design of aerated reactors for recombinant cells.
研究了溶解氧冲击对含有质粒pKN401的重组大肠杆菌细胞稳定性的影响。在含和不含氨苄青霉素的培养基中进行的对照分批实验中，重组细胞是稳定的。然而，在诱导溶解氧冲击的条件下，这些重组细胞高度不稳定。这些结果对重组细胞曝气反应器的设计具有启示意义。
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