接受抗肿瘤坏死因子-α抗体治疗的类风湿性关节炎患者的蛋白质生物标志物质谱分析。
Protein biomarker analysis by mass spectrometry in patients with rheumatoid arthritis receiving anti-tumor necrosis factor-alpha antibody therapy.
作者信息
Sekigawa I, Yanagida M, Iwabuchi K, Kaneda K, Kaneko H, Takasaki Y, Jung G, Sone S, Tanaka Y, Ogawa H, Takamori K
机构信息
Department of Internal Medicine, Juntendo University Urayasu Hospital, Chiba, Japan.
出版信息
Clin Exp Rheumatol. 2008 Mar-Apr;26(2):261-7.
OBJECTIVE
To investigate the mechanism of action of anti-tumor necrosis factor-alpha (TNF-alpha) antibody in patients with rheumatoid arthritis (RA), we analyzed serum or plasma proteins by mass spectrometry system.
METHODS
Ten RA patients who received treatment with anti-TNF-alpha antibody were studied. Samples obtained before and after therapy were analyzed by a two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) system after pretreatment by a recently developed method to remove high molecular weight proteins.
RESULTS
Using this system, certain proteins were identified after treatment with anti-TNF-alpha antibody, including proteins related to the TNF-alpha-mediated pathway for nuclear factor kappa B (NF-kappaB) activation and/or to the metabolism (including regeneration) of articular cartilage.
CONCLUSION
Our mass spectrometry system appears to be useful for proteomic analysis. The efficacy of anti-TNF-alpha antibody therapy for RA may be related to various consequence of the inhibition of TNF-alpha activity.
目的
为了研究抗肿瘤坏死因子-α（TNF-α）抗体在类风湿关节炎（RA）患者中的作用机制，我们通过质谱系统分析了血清或血浆蛋白。
方法
对10例接受抗TNF-α抗体治疗的RA患者进行研究。在采用最近开发的去除高分子量蛋白的方法进行预处理后，通过二维液相色谱串联质谱（2D LC-MS/MS）系统对治疗前后获得的样本进行分析。
结果
使用该系统，在用抗TNF-α抗体治疗后鉴定出了某些蛋白，包括与TNF-α介导的核因子κB（NF-κB）激活途径和/或关节软骨代谢（包括再生）相关的蛋白。
结论
我们的质谱系统似乎可用于蛋白质组学分析。抗TNF-α抗体治疗RA的疗效可能与TNF-α活性抑制的各种结果有关。
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