Lehrbach Nicolas J, Miska Eric A
Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge CB2 1QN, UK.
Brief Funct Genomic Proteomic. 2008 May;7(3):228-35. doi: 10.1093/bfgp/eln024. Epub 2008 Jun 19.
MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression in many eukaryotes. miRNAs were first discovered in Caenorhabditis elegans by Victor Ambros' laboratory in 1993. At the same time Gary Ruvkun's laboratory identified the first miRNA target gene. Together, these two seminal discoveries identified a novel mechanism of post-transcriptional gene regulation that has been recognized as important for development, physiology and pathology of many organisms. Here we discuss how functional genomic, computational and proteomic approaches complement classical genetic analyses to unravel miRNA biology in C. elegans.
微小RNA（miRNA）是一类短链非编码RNA，可在许多真核生物中调节基因表达。1993年，维克托·安布罗斯实验室在秀丽隐杆线虫中首次发现了miRNA。与此同时，加里·鲁夫昆实验室鉴定出了首个miRNA靶基因。这两项开创性的发现共同揭示了一种新的转录后基因调控机制，该机制已被认为对许多生物体的发育、生理和病理过程都很重要。在此，我们将讨论功能基因组学、计算生物学和蛋白质组学方法如何与经典遗传学分析相互补充，以阐明秀丽隐杆线虫中的miRNA生物学。
