Department of Applied Microbiology, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, Poland.
Biotechnol Bioeng. 1988 Sep 5;32(6):771-6. doi: 10.1002/bit.260320608.
Autohydrolysis and ethanol-alkali pulping were used as pretreatment methods of wheat straw for its subsequent saccharification by Trichoderma reesei cellulase. The basic hydrolysis parameters, i.e., reaction time, pH, temperature, and enzyme and substrate concentration, were optimized to maximize sugar yields from ethanol-alkali modified straw. Thus, a 93% conversion of 2.5% straw material to sugar syrup containing 73% glucose was reached in 48 h using 40 filter paper units/g hydrolyzed substrate. The pretreated wheat straw was then fermented to ethanol at 43 degrees C in the simultaneous saccharification and fermentation (SSF) process using T. reesei cellulase and Kluyveromyces fragilis cells. From 10% (w/v) of chemically treated straw (dry matter), 2.4% (w/v) ethanol was obtained after 48 h. When the T. reesei cellulase system was supplemented with beta-glucosidase from Aspergillus niger, the ethanol yield in the SSF process increased to 3% (w/v) and the reaction time was shortened to 24 h.
采用自水解和乙醇-碱预处理小麦秸秆，然后用里氏木霉纤维素酶对其进行后续糖化。优化了基本水解参数，如反应时间、pH 值、温度、酶和底物浓度，以最大限度地提高乙醇-碱改性秸秆的糖产量。因此，在 48 小时内，使用 40 个滤纸单位/克水解底物，将 2.5%的秸秆材料转化为含有 73%葡萄糖的糖浆，转化率达到 93%。然后，将预处理的小麦秸秆在 43°C 下进行同步糖化和发酵（SSF）过程，用里氏木霉纤维素酶和脆弱拟杆菌细胞发酵生产乙醇。从 10%（w/v）的化学处理秸秆（干物质）中，经过 48 小时可获得 2.4%（w/v）的乙醇。当 T. reesei 纤维素酶系统中补充来自黑曲霉的β-葡萄糖苷酶时，SSF 过程中的乙醇产量增加到 3%（w/v），反应时间缩短至 24 小时。
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