Cao Wei, Wang Mingzhong, Wang Xiaoying, Liu Xiumei
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2008 Mar;37(2):245-8.
The traditional methods of bacteria isolation, cultivation and identification are time-consuming, which can't meet the needs of the control and prevention of food-borne diseases. Recently, various kinds of rapid methods for food-borne pathogenic bacteria detection have emerged with the prompt development of nucleic acid testing technology. The application studies on polymerase chain reaction and the techniques derived from it, nucleic acid isothermal amplification, oligonucleotide microarray, immunomagnetic separation and DNA biosensing on food-borne pathogenic bacteria including Salmonella, Staphylococcus aureus and Enterohemorrhagic Escherchia coli, etc. were reviewed.
传统的细菌分离、培养和鉴定方法耗时较长,无法满足食源性疾病防控的需求。近年来,随着核酸检测技术的迅速发展,出现了各种食源性病原体细菌的快速检测方法。本文综述了聚合酶链反应及其衍生技术、核酸等温扩增、寡核苷酸微阵列、免疫磁分离和DNA生物传感技术在沙门氏菌、金黄色葡萄球菌和肠出血性大肠杆菌等食源性病原体细菌检测中的应用研究。