编码一种GARP蛋白的卷叶9调控水稻叶片远轴面细胞命运。
ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice.
作者信息
Yan Song, Yan Chang-Jie, Zeng Xiu-Hong, Yang Ya-Chun, Fang Yu-Wei, Tian Chun-Yan, Sun Ya-Wei, Cheng Zhu-Kuan, Gu Ming-Hong
机构信息
The Key Laboratory of Plant Functional Genomics, Ministry of Education of China, Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, 12 East Wenhui Road, Jiangsu 225009, People's Republic of China.
出版信息
Plant Mol Biol. 2008 Oct;68(3):239-50. doi: 10.1007/s11103-008-9365-x. Epub 2008 Jul 2.
Leaves, the collective organ produced by the shoot apical meristem (SAM), are polarized along their adaxial-abaxial axis. In this study, we characterized two rice (Oryza sativa) allelic rolled-leaf mutants, rolled leaf 9-1 (rl9-1) and rl9-2, which display very similar phenotypes with completely adaxialized leaves and malformed spikelets. We cloned the RL9 gene by way of a map-based cloning strategy. Molecular studies have revealed that RL9 encodes a GARP protein, an orthologue of Arabidopsis KANADIs. RL9 is mainly expressed in roots, leaves, and flowers. The transient expression of a RL9-GFP (green fluorescent protein) fusion protein has indicated that RL9 protein is localized in the nucleus, suggesting that RL9 acts as a putative transcription factor.
叶片是由茎尖分生组织（SAM）产生的集合器官，沿其近轴-远轴轴向极化。在本研究中，我们对两个水稻（Oryza sativa）等位基因卷叶突变体卷叶9-1（rl9-1）和rl9-2进行了表征，它们表现出非常相似的表型，叶片完全近轴化且小穗畸形。我们通过图位克隆策略克隆了RL9基因。分子研究表明，RL9编码一种GARP蛋白，是拟南芥KANADIs的同源物。RL9主要在根、叶和花中表达。RL9-绿色荧光蛋白（GFP）融合蛋白的瞬时表达表明，RL9蛋白定位于细胞核，这表明RL9作为一种假定的转录因子发挥作用。
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