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L 视锥细胞和 M 视锥细胞分离视网膜电图:发光二极管与阴极射线管刺激

L- and M-cone isolating ERGs: LED versus CRT stimulation.

作者信息

Murray I J, Kremers J, Parry N R A

机构信息

Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.

出版信息

Vis Neurosci. 2008 May-Jun;25(3):327-31. doi: 10.1017/S095252380808036X.

Abstract

Using double silent substitution, it is possible to generate L-cone and M-cone isolating electroretinograms (ERGs) on a CRT. A major limitation of the technique is that the depth of modulation of cone classes is limited by the restricted luminance of the phosphors and their spectral overlap. To address this problem we have ported the technique to a four-color LED Ganzfeld stimulus (Diagnosis ColorDome). This allows higher retinal illuminances, higher contrasts, and triple silent substitution. With careful control over the retinal area stimulated, we show that the same data can be recorded from both CRT and LED stimuli when luminance, size and cone contrast are kept constant. Importantly, the different temporal profiles of the two devices do not influence the ERG amplitude and phase plots. We present data over a much wider range of luminances (up to about 10,000 trolands) and contrasts with the LED stimulator than previously reported with CRT screens. We conclude that the close resemblance between data obtained with an LED stimulator and with a CRT screen indicate that the differences have a purely physiological origin.

摘要

通过双沉默替换,可以在阴极射线管(CRT)上生成分离L视锥细胞和M视锥细胞的视网膜电图(ERG)。该技术的一个主要限制是,视锥细胞类别的调制深度受到荧光粉有限亮度及其光谱重叠的限制。为了解决这个问题,我们已将该技术移植到四色发光二极管全视野刺激器(诊断用ColorDome)上。这使得视网膜照度更高、对比度更高,并且可以进行三重沉默替换。通过仔细控制受刺激的视网膜区域,我们表明,当亮度、大小和视锥细胞对比度保持恒定时,使用CRT和发光二极管刺激器都可以记录到相同的数据。重要的是,这两种设备不同的时间特性不会影响ERG的幅度和相位图。与之前使用CRT屏幕报道的相比,我们展示了在发光二极管刺激器下,在更宽的亮度范围(高达约10,000特罗兰)和对比度下的数据。我们得出结论,发光二极管刺激器和CRT屏幕获得的数据非常相似,这表明差异纯粹源于生理原因。

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