三氧化二砷体外诱导视网膜母细胞瘤细胞凋亡及其可能机制

[Arsenic trioxide induced apoptosis in retinoblastoma cells in vitro and its possible mechanism].

作者信息

Li Yun, Tang Luo-Sheng, Shen Hong-Wei

机构信息

Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Jun;33(6):476-80.

PMID:18599993

Abstract

OBJECTIVE

To explore the effect of arsenic trioxide on the apoptosis of retinoblastoma cell line HXO-RB(44) and the possible mechanism.

METHODS

The effect of arsenic trioxide on the proliferation of retinoblastoma cell line HXO-RB(44) was observed by MTT colorimetric assay; the apoptosis of the HXO-RB(44) was examined by AO/EB staining and flow cytometry analysis (Annexin V+ PI staining); caspase-3 activity and bcl-2/bax expression in the HXO-RB(44) were detected by cpp32 colorimetric assay kit and Western blot.

RESULTS

Arsenic trioxide inhibited the proliferation of HXO-RB(44) cell in dose and duration-dependent manner in vitro; arsenic trioxide significantly increased the apoptosis in HXO-RB(44) cells. The activation of caspase-3 was increased, and the rate of bcl-2/bax was down-regulated in the HXO-RB(44) cells processed with arsenic trioxide.

CONCLUSION

Arsenic trioxide can inhibit the proliferation of retinoblastoma cell HXO-RB(44) in vitro by apoptosis induction. The apoptosis induction is possibly related to the caspase-3 activation and bcl-2/bax down-regulation.

摘要

目的

探讨三氧化二砷对视网膜母细胞瘤细胞系HXO-RB(44)凋亡的影响及其可能机制。

方法

采用MTT比色法观察三氧化二砷对视网膜母细胞瘤细胞系HXO-RB(44)增殖的影响；采用AO/EB染色及流式细胞术分析（Annexin V+PI染色）检测HXO-RB(44)细胞的凋亡情况；采用cpp32比色法试剂盒及Western blot检测HXO-RB(44)细胞中caspase-3活性及bcl-2/bax表达。