体外牛大脑皮层突触膜蛋白的磷酸化。用曲拉通X-100对底物和蛋白激酶活性进行分配。
Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100.
作者信息
Dunkley P R, Holmes H, Rodnight R
出版信息
Biochem J. 1976 Sep 1;157(3):661-6. doi: 10.1042/bj1570661.
Abstract
Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.
摘要
来自牛大脑皮层的突触膜碎片含有基础的和环磷酸腺苷（cAMP）刺激的蛋白激酶活性，可催化内源性底物的磷酸化。用曲拉通X-100提取膜碎片，可溶解不到20%的激酶活性，并使大部分内源性底物留在不溶性部分。
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