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人NUDT16的结晶及晶体学分析

Crystallization and crystallographic analysis of human NUDT16.

作者信息

Zhang Jie, Gao Feng, Zhang Qianmin, Chen Qianying, Qi Jianxun, Yan Jinghua

机构信息

Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jul 1;64(Pt 7):639-40. doi: 10.1107/S1744309108016928. Epub 2008 Jun 11.

DOI:10.1107/S1744309108016928
PMID:18607096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2443980/
Abstract

Human NUDT16, a decapping enzyme belonging to the Nudix superfamily, plays a pivotal role in U8 snoRNA stability. Recombinant NUDT16 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals, which diffracted to 2.10 A resolution, belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.47, b = 79.32, c = 97.20 A. The Matthews coefficient and the solvent content were calculated to be 1.92 A(3) Da(-1) and 35.84%, respectively, for two molecules per asymmetric unit.

摘要

人NUDT16是一种属于Nudix超家族的去帽酶，在U8小核仁RNA（snoRNA）稳定性中起关键作用。采用悬滴气相扩散法使在大肠杆菌中表达的重组NUDT16结晶。这些晶体的衍射分辨率为2.10 Å，属于空间群P2(1)2(1)2(1)，晶胞参数a = 44.47、b = 79.32、c = 97.20 Å。对于每个不对称单元中的两个分子，计算得到的马修斯系数和溶剂含量分别为1.92 Å(3) Da(-1)和35.84%。

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