Chen Fei, Eriksson Per, Whatling Carl, Vaage Jarle, Valen Guro
King Gustaf V:s Research Institute, Karolinska Institute, Stockholm, Sweden.
Scand Cardiovasc J. 2008 Oct;42(5):327-36. doi: 10.1080/14017430801919540.
To investigate whether human veins responded to surgical handling with acute remodelling by measuring matrix metalloproteinase 9 (MMP9) expression and activity.
Saphenous veins were collected from 24 patients (12 stable angina, 12 unstable angina) undergoing coronary artery bypass grafting. Expression of MMP9 and its regulators (plasminogen activators, plasminogen activator inhibitor-1, tissue inhibitor of metalloproteinase-1) was evaluated by semiquantitative RT-PCR in veins sampled at the start of and after surgical preparation, while protein was detected by western blotting. The proteolytic activity of MMP9 was analyzed by zymography.
Gene (p=0.01) and protein (p=0.001) expression of MMP9 increased after surgical manipulation of vein grafts in all patients, accompanied by increased pro-MMP9 (p=0.04), but not active MMP9 (p=0.6). Grafts from stable patients had increased gene (p=0.05) and protein (p=0.006) expression, as well as increased pro- (p=0.04) and active (p=0.04) MMP9. Grafts from unstable patients increased only in MMP9 protein expression (p=0.05). The MMP9 regulators were unchanged.
Surgical handling of vein grafts increased expression and activity of MMP9. However, the surgery-induced increase was attenuated in veins from unstable patients.
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