两种昆虫细胞系在无血清培养中用于杆状病毒表达系统的特性

Properties of two insect cell lines useful for the baculovirus expression system in serum-free culture.

作者信息

Sugiura T, Amann E

机构信息

Laboratory for Bone Research, Drug Discovery Research Laboratories, Pharma Research and Development Division, Hoechst Japan Limited 3-2, 1-Chome, Minamidai Kawagoe-city, Saitama 350-11, Japan.

出版信息

Biotechnol Bioeng. 1996 Aug 20;51(4):494-9. doi: 10.1002/(SICI)1097-0290(19960820)51:4<494::AID-BIT13>3.0.CO;2-I.

DOI:10.1002/(SICI)1097-0290(19960820)51:4<494::AID-BIT13>3.0.CO;2-I

PMID:18629802

Abstract

The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. Sf9 cells in SF900II medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medium consumed all the nutrients examined, including sucrose. The productivity of a recombinant glycoprotein, OSF-2, by Tn5 cells, was moderate in both monolayer and spinner cultures, but the ability to secrete it was compromised in the former case. Relative to the Tn5 cultures, Sf9 produced 30-fold more OSF-2 in either culture mode. (c) 1996 John Wiley & Sons, Inc.

摘要

在无血清培养条件下，对Sf9和Tn5昆虫细胞的特性进行了比较分析。在SF900II培养基中的Sf9细胞在病毒感染前后显然都将蔗糖作为主要营养物质，产生少量乳酸和氨。在Excell 401培养基中的Tn5细胞消耗了所有检测的营养物质，包括蔗糖。Tn5细胞产生重组糖蛋白OSF-2的能力在单层培养和转瓶培养中都处于中等水平，但在前一种情况下其分泌能力受到损害。相对于Tn5培养物，Sf9在两种培养模式下产生的OSF-2都多30倍。(c) 1996约翰·威利父子公司。