Amirzargar Ali Akbar, Naroueynejad Mehrnaz, Khosravi Farideh, Dianat Seyed Saied, Rezaei Nima, Mytilineos Joannis, Nikbin Behrouz
Immunogenetic Laboratory, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Iran.
Eur Cytokine Netw. 2008 Jun;19(2):104-12. doi: 10.1684/ecn.2008.0122. Epub 2008 Jun 1.
Cytokines are important immunomodulatory molecules involved in immune responses against microorganisms; they also have an important role in the setting of immune system disorders. Cytokine single nucleotide polymorphisms have been extensively studied in different, normal populations as well as in association with disease. Cytokine gene polymorphisms are potentially important as genetic predictors of disease susceptibility, clinical outcome, and as a tool for anthropological studies. In this study, samples have been collected from 455 healthy individuals located in different regions of Iran (Tehran, Yazd, Sistan and Balochistan). Allele and genotype frequencies of cytokine SNP, including: IL-1alpha, IL-1beta, IL-1R, IL-1RA, IL-2, IL-4, IL-4RA, IL-6, IL-10, IL-12, TNF-alpha, TGF-beta and IFN-gamma were investigated, using the PCR-SSP method. Allele frequencies in Tehran and Yazd populations were similar, except for TGF-beta. Allele frequencies in Sistani & Baloch populations were similar at all positions, except for IL-1beta at position of -511 and IFN-gamma genes at position UTR5644; there were some differences in allele frequencies comparing these populations with the Yazd population, including: IL-4, IL-6, IL-10, TGF-beta and TNF-alpha. Although some significant differences were observed for some cytokines, it seems that the cytokine gene polymorphism profile of the Iranian population is similar to that of Caucasians, particularly the Italian population.
细胞因子是参与针对微生物的免疫反应的重要免疫调节分子;它们在免疫系统疾病的发生中也起着重要作用。细胞因子单核苷酸多态性已在不同的正常人群中以及与疾病的关联中得到广泛研究。细胞因子基因多态性作为疾病易感性、临床结果的遗传预测指标以及人类学研究工具具有潜在重要性。在本研究中,从伊朗不同地区(德黑兰、亚兹德、锡斯坦和俾路支斯坦)的455名健康个体中采集了样本。使用PCR-SSP方法研究了细胞因子单核苷酸多态性的等位基因和基因型频率,包括:IL-1α、IL-1β、IL-1R、IL-1RA、IL-2、IL-4、IL-4RA、IL-6、IL-10、IL-12、TNF-α、TGF-β和IFN-γ。除TGF-β外,德黑兰和亚兹德人群的等位基因频率相似。锡斯坦和俾路支人群在所有位置的等位基因频率相似,除了-511位的IL-1β和UTR5644位的IFN-γ基因;与亚兹德人群相比,这些人群在等位基因频率上存在一些差异,包括:IL-4、IL-6、IL-10、TGF-β和TNF-α。尽管在某些细胞因子上观察到了一些显著差异,但伊朗人群的细胞因子基因多态性谱似乎与高加索人群,特别是意大利人群相似。