Knell J D, Summers M D
Department of Entomology, Texas A&M University, College Station, Texas 77843, USA.
Virology. 1981 Jul 15;112(1):190-7. doi: 10.1016/0042-6822(81)90624-3.
Spodoptera frugiperda nuclear polyhedrosis virus (SfMNPV was obtained from four different sources and each isolate was cultured in IPLB-SF21 cells. Viral DNA from each isolate was analyzed with EcoR1, Xho1, and BamH1 restriction endonucleases. All four isolates could be distinguished on the basis of minor differences in EcoR1 patterns. Submolar DNA restriction fragments were seen in all isolates indicating that each isolate consisted of a mixture of different genotypes. To investigate this, two of the isolates were plaque purified. Of the total of 13 clones isolated, the DNA from 5 of these had distinctly different EcoR1 restriction endonuclease patterns. The five cloned variants were further analyzed with Xho1, BamH1, and Sac1. Three of the five clones had fragments which comigrated with submolar fragments seen in the wild isolates. Thus, the submolar fragments observed in digests of the DNA from two wild isolates of SfMNPV were due to a mixture of genetic variants.
草地贪夜蛾核型多角体病毒（SfMNPV）来自四个不同的来源，每个分离株都在IPLB - SF21细胞中培养。用EcoR1、Xho1和BamH1限制性内切酶分析每个分离株的病毒DNA。根据EcoR1图谱的微小差异，可以区分所有四个分离株。在所有分离株中都观察到亚摩尔DNA限制性片段，这表明每个分离株都由不同基因型的混合物组成。为了研究这一点，对其中两个分离株进行了噬菌斑纯化。在总共分离出的13个克隆中，其中5个克隆的DNA具有明显不同的EcoR1限制性内切酶图谱。对这五个克隆变体进一步用Xho1、BamH1和Sac1进行分析。五个克隆中的三个具有与野生分离株中观察到的亚摩尔片段共迁移的片段。因此，在SfMNPV两个野生分离株的DNA消化物中观察到的亚摩尔片段是由于遗传变体的混合物。
