Biochemical properties of a persistent nonoccluded baculovirus isolated from Heliothis zea cells. - Suppr | 超能文献

Biochemical properties of a persistent nonoccluded baculovirus isolated from Heliothis zea cells.

作者信息

Langridge W H

机构信息

Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, New York 14853, USA.

出版信息

Virology. 1981 Jul 30;112(2):770-4. doi: 10.1016/0042-6822(81)90324-x.

DOI:10.1016/0042-6822(81)90324-x

Abstract

A persistent nonoccluded rod-shaped nuclear virus of Heliothis zea (IMC-Hz-1-NOV) was isolated from Trichoplusia ni cells (Tn-368) by sucrose density gradient centrifugation. The molecular weights of IMC-Hz-1-NOV structural proteins were determined by SDS-polyacrylamide gel electrophoresis. Separation of IMC-Hz-1-NOV DNA restriction fragments by agarose gel electrophoresis gave an approximate DNA molecular weight of 132 x 10(6). Digestion of double-stranded IMC-Hz-1-NOV DNA with HindIII and BamHI revealed a cleavage pattern different from the patterns of H. zea NPV and H. armigera NPV.

摘要

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