从一种真核藻类中分离出一种病毒样颗粒并进行部分化学特性分析。

Isolation and partial chemical characterization of a virus-like particle from a eukaryotic alga.

作者信息

Stanker L H, Hoffman L R, MacLeod R

机构信息

Department of Botany, Development, University of Illinois, Urbana, Illinois 61801, USA.

出版信息

Virology. 1981 Oct 31;114(2):357-69. doi: 10.1016/0042-6822(81)90217-8.

DOI:10.1016/0042-6822(81)90217-8

PMID:18635097

Abstract

A virus-like particle (VLP) associated with the green alga Cylindrocapsa geminella has been purified and partially characterized. The Cylindrocapsa VLP (C-VLP) has a polygonal profile in sectional view, ranges in diameter from 200 to 230 nm, and has a complex, multilayered capsid. Purified VLPs, when analyzed by SDS-polyacrylamide gel electrophoresis, contained at least 10 proteins ranging in molecular weight from 10,000 to 160,000. The C-VLP contains DNA, most probably double-stranded, having an estimated molecular weight of 175-190 x 10(6). Intracellular VLPs were only observed within single-celled germlings and all cultures examined produced the VLP. Generally, only 4-6% of the single-celled germlings contained VLPs, which resulted in cell death. The physicochemical and biological characteristics of the C-VLP support the conclusion that the C-VLP is, in fact, a virus.

摘要

一种与绿藻双生柱孢藻相关的病毒样颗粒（VLP）已被纯化并进行了部分特性分析。柱孢藻VLP（C-VLP）在截面视图中呈多边形轮廓，直径范围为200至230纳米，具有复杂的多层衣壳。通过SDS-聚丙烯酰胺凝胶电泳分析纯化的VLP时，含有至少10种分子量在10,000至160,000之间的蛋白质。C-VLP含有DNA，很可能是双链的，估计分子量为175 - 190×10⁶。细胞内VLP仅在单细胞幼体中观察到，并且所检查的所有培养物都产生VLP。通常，只有4 - 6%的单细胞幼体含有VLP，这导致细胞死亡。C-VLP的物理化学和生物学特性支持了C-VLP实际上是一种病毒的结论。