番木瓜花叶病毒外壳蛋白的一级结构。

The primary structure of papaya mosaic virus coat protein.

作者信息

Short M N, Turner D S, March J F, Pappin D J, Parente A, Davies J W

机构信息

Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, United Kingdom.

出版信息

Virology. 1986 Jul 15;152(1):280-3. doi: 10.1016/0042-6822(86)90395-8.

DOI:10.1016/0042-6822(86)90395-8

PMID:18640641

Abstract

The amino acid sequence of the coat protein of the potexvirus papaya mosaic virus (PMV) has been determined, using a combination of the Edman degradation procedure (manual and automated) and mass spectrometry. The amino acid sequence is compared with that of the coat protein of potato virus X (PVX) as reported (S. Yu. Morozov, V. M. Zakhariev, B. K. Chernov, V. S. Prasolov, Yu. V. Kozlov, J. G. Atabekov, and K. G. Skyabin, Dokl. Acad. Nauk, SSSR 271, 211-215,1983). PMV has 73 of 211 amino acid residues in common with PVX and has 25 fewer residues in the polypeptide chain. Also 10 of a possible 16 proline residues are in similar positions in both proteins, including a sequence of 3 prolines at the carboxyl end. Furthermore, the 2 cysteine residues in PMV correspond with 2 of the 3 cysteines in PVX.

摘要

利用埃德曼降解法（手动和自动）与质谱分析法相结合，已确定了马铃薯X病毒属番木瓜花叶病毒（PMV）外壳蛋白的氨基酸序列。将该氨基酸序列与已报道的马铃薯X病毒（PVX）外壳蛋白的氨基酸序列进行了比较（S. Yu. 莫罗佐夫、V. M. 扎哈里耶夫、B. K. 切尔诺夫、V. S. 普拉索洛夫、Yu. V. 科兹洛夫、J. G. 阿塔贝科夫和K. G. 斯基亚宾，《苏联科学院报告》271, 211 - 215, 1983年）。PMV与PVX共有211个氨基酸残基中的73个，且其多肽链中的残基少25个。在两种蛋白质中，可能的16个脯氨酸残基中有10个处于相似位置，包括羧基末端的3个脯氨酸序列。此外，PMV中的2个半胱氨酸残基与PVX中3个半胱氨酸中的2个相对应。