直接应用于脑膜炎奈瑟菌病患者临床样本的可变数目串联重复聚合酶链反应。
Direct application of variable number tandem repeats polymerase chain reaction in clinical samples obtained from patients with meningococcal disease.
机构信息
National Meningitis Reference Laboratory, National School of Public Health, 11522 Athens, Greece.
出版信息
Diagn Microbiol Infect Dis. 2010 Jan;66(1):124-7. doi: 10.1016/j.diagmicrobio.2008.05.015. Epub 2008 Jul 21.
Abstract
A nested variable number tandem repeats (VNTR) polymerase chain reaction (PCR) technique was developed for the direct typing of meningococcal PCR-positive clinical samples. The system was evaluated on a panel of 43 clinical samples and isolates positive for Neisseria meningitidis. The results revealed that VNTR-PCR can be used directly in clinical samples, allowing fine typing of N. meningitidis.
摘要
建立了一种巢式可变数目串联重复（VNTR）聚合酶链反应（PCR）技术，用于直接对脑膜炎球菌 PCR 阳性临床标本进行分型。该系统在 43 份脑膜炎奈瑟菌阳性的临床标本和分离株上进行了评估。结果表明，VNTR-PCR 可直接用于临床标本，可对脑膜炎奈瑟菌进行精细分型。
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