Bondan Eduardo Fernandes, Lallo Maria Anete, Graça Dominguita Lühers
Universidade Paulista, São Paulo, SP, Brazil.
Arq Neuropsiquiatr. 2008 Jun;66(2B):378-84. doi: 10.1590/s0004-282x2008000300019.
The ethidium bromide-demyelinating model (EB) was used to study remyelination in the brainstem under the use of cyclosporine (CsA). Wistar rats were submitted to intracisternal injection of 0.1% EB or 0.9% saline solution, and others were taken as histologic controls (group I). Within those injected with EB, some have not received immunosuppressive treatment (II); some were treated by intraperitonial route with CsA (III.E-10 mg/kg/day). Rats from group III.C were injected with saline solution and treated with CsA. The animals were perfused from 15 to 31 days post-injection collecting brainstem sections for light and transmission electron microscopy studies. After EB injection it was noted the presence of macrophages and non-degraded myelin debris, demyelinated axons, oligodendrocyte or Schwann cell remyelinated axons, groups of infiltrating pial cells, hypertrophic astrocytes and few lymphocytes. Tissue repair of EB-induced lesions in group III.E was similar to that of group II, but with the presence of a higher density of oligodendrocytes near remyelinating areas.
采用溴化乙锭脱髓鞘模型（EB），在使用环孢素（CsA）的情况下研究脑干的髓鞘再生。将Wistar大鼠经脑池内注射0.1% EB或0.9%盐溶液，其他大鼠作为组织学对照（I组）。在注射EB的大鼠中，一些未接受免疫抑制治疗（II组）；一些经腹腔途径用CsA治疗（III.E组 - 10 mg/kg/天）。III.C组大鼠注射盐溶液并用CsA治疗。在注射后15至31天对动物进行灌注，收集脑干切片用于光镜和透射电镜研究。注射EB后，观察到存在巨噬细胞和未降解的髓鞘碎片、脱髓鞘轴突、少突胶质细胞或施万细胞再髓鞘化的轴突、浸润的软膜细胞群、肥大的星形胶质细胞和少量淋巴细胞。III.E组中EB诱导损伤的组织修复与II组相似，但在再髓鞘化区域附近少突胶质细胞密度更高。
