人成肌细胞可调节抗原呈递细胞的功能。

Human myoblasts modulate the function of antigen-presenting cells.

作者信息

Schwab Nicholas, Waschbisch Anne, Wrobel Barbara, Lochmüller Hanns, Sommer Claudia, Wiendl Heinz

机构信息

Department of Neurology, University of Wuerzburg, Germany.

出版信息

J Neuroimmunol. 2008 Aug 30;200(1-2):62-70. doi: 10.1016/j.jneuroim.2008.06.012. Epub 2008 Jul 21.

DOI:10.1016/j.jneuroim.2008.06.012

PMID:18644633

Abstract

Muscle biopsy specimens of myositis patients were analyzed for the presence of dendritic cells (DC) and macrophages (MPh) by immunohistochemistry. The interaction of DC and myoblasts (MB) was studied by coculture and effects on DC phenotype and function were assessed by flow cytometry and T-cell proliferation assays. Effects of MB-lysates on the phagocytic capacity of MPh were analyzed in bead-incorporation assays. Myositis specimens revealed a tendency towards more immature DC. MB modulated the maturation state of DC and DC recovered from MB-coculture had an inhibitory effect on T-cell proliferation. MB-lysates strongly stimulated MPh phagocytosis. Hypothetically, MB might modulate APC, counterbalancing immune-mediated damage.

摘要

通过免疫组织化学分析了肌炎患者的肌肉活检标本中树突状细胞（DC）和巨噬细胞（MPh）的存在情况。通过共培养研究了DC与成肌细胞（MB）的相互作用，并通过流式细胞术和T细胞增殖试验评估了对DC表型和功能的影响。在珠子掺入试验中分析了MB裂解物对MPh吞噬能力的影响。肌炎标本显示出倾向于更多不成熟DC的趋势。MB调节DC的成熟状态，从MB共培养中恢复的DC对T细胞增殖具有抑制作用。MB裂解物强烈刺激MPh吞噬作用。假设MB可能调节抗原呈递细胞，抵消免疫介导的损伤。