Qu Liang-Huan, Sun Meng-Xiang
Key Laboratory of the Ministry of Education for the Development of Biology, College of Life Science, Wuhan University, Wuhan, People's Republic of China.
Protoplasma. 2008;233(1-2):51-9. doi: 10.1007/s00709-008-0304-0. Epub 2008 Jul 22.
A convenient experimental system was established to test how cells derived from higher-plant internal tissues respond to mechanical stimulation. Short-term culture of tobacco ovules in vitro led to the generation of bar-shaped cells from the parenchyma tissue of the ovule funicle. These cells are still connected to the mother tissue and are almost undifferentiated. The cells are translucent, and one end protrudes from the funicle, making them easy to manipulate and observe. Mechanical stimulation tests performed on these cells indicated that the cells are less sensitive to mechanical stimulation than epidermal hair cells but still possess the ability to respond to stimulation. Interestingly, the cells showed a cytoplasmic compartmental response to the stimulation. The nucleus, some plastids, and mitochondria were organized into a responsive unit that moved in unison to the stimulated sites, whereas most of the other organelles were not notably influenced by the stimulation. This suggests that the cytoplasm is highly organized and functionally divided in response to environmental stimulation.
建立了一个便捷的实验系统，以测试源自高等植物内部组织的细胞如何对机械刺激作出反应。烟草胚珠的体外短期培养导致胚珠柄薄壁组织产生棒状细胞。这些细胞仍与母组织相连，几乎未分化。细胞是半透明的，一端从柄部突出，便于操作和观察。对这些细胞进行的机械刺激测试表明，它们对机械刺激的敏感性低于表皮毛细胞，但仍具有对刺激作出反应的能力。有趣的是，细胞对刺激表现出细胞质区室反应。细胞核、一些质体和线粒体被组织成一个反应单元，该单元一致地移动到受刺激部位，而大多数其他细胞器并未受到刺激的显著影响。这表明细胞质在响应环境刺激时高度有序且功能分化。
