[利用携带小鼠金属硫蛋白-I基因的转基因聚球藻属PCC 7002对废水中重金属进行净化]

[Decontamination of heavy metals in wastewater by transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene].

作者信息

Zeng Wen-Lu, Zhao Fei-Fei, Cao Zhao-Gen, Ru Bing-Gen

机构信息

Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

出版信息

Huan Jing Ke Xue. 2008 Mar;29(3):738-44.

PMID:18649537

Abstract

The growth characteristics and the decontamination of heavy metals in analogous heavy metals wastewater by transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene were studied. The results show that transgenic Synechococcus sp. PCC 7002 not only has a higher tolerance to heavy metals, but also has a higher growth rate than wild strain. The concentration of Cd2+, Pb2+ and Hg2+ decreases with the progress of cultivation, and its maximum decreasing extent occurs at 1 - 3 day. After three days of cultivation, the absorption of Cd2+, Pb2+ and Hg2+ by transgenic Synechococcus sp. PCC 7002 is 10.75, 58.89 and 112.61 mg g(-1) of dried cells respectively, which is 3.16, 2.18 and 100.45 times higher than wild cells. The mono-factor and overall model developed fit the experiment data well.

摘要

研究了转小鼠金属硫蛋白-I基因的聚球藻属PCC 7002对模拟重金属废水中重金属的生长特性及去除效果。结果表明，转基因聚球藻属PCC 7002不仅对重金属具有较高的耐受性，而且生长速率高于野生菌株。随着培养进程，Cd2+、Pb2+和Hg2+浓度降低，最大降幅出现在第1至3天。培养三天后，转基因聚球藻属PCC 7002对Cd2+、Pb2+和Hg2+的吸收量分别为10.75、58.89和112.61 mg g(-1)干细胞，分别是野生细胞的3.16、2.18和100.45倍。所建立的单因素和整体模型与实验数据拟合良好。