Li Lina, Wu Gang, Sun Jin, Li Bai, Li Yufeng, Chen Chunying, Chai Zhifang, Iida Atsuo, Gao Yuxi
Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
J Toxicol Environ Health A. 2008;71(18):1266-9. doi: 10.1080/15287390802216041.
Mercury (Hg)-, selenium (Se)-, and arsenic (As)- containing proteins in liver tissues of bighead carp and grass carp sampled from a mercury-polluted area of Wanshan, Guizhou Province, China, were separated by thin-layer isoelectric focusing. The relative content of Hg, Se, and As in protein bands was measured with synchrotron radiation x-ray fluorescence. The results indicated that there were at least 3 Hg-containing bands with pI 3.7, 4.8, and 6.2 in liver of bighead carp and 1 Hg-containing band with pI 6.2 in grass carp. Se and As were found in the Hg-containing bands 3.7 in bighead carp and 6.2 in grass carp. The bands may be corresponding to the antagonistic effect of Se against the toxicity of Hg and As. In addition, Hg and As often coexist in the same band, suggesting that the two elements may be involved in the same metabolic processes.
从中国贵州省万山汞污染地区采集的鳙鱼和草鱼肝脏组织中，含汞（Hg）、硒（Se）和砷（As）的蛋白质通过薄层等电聚焦进行分离。利用同步辐射X射线荧光测定蛋白质条带中Hg、Se和As的相对含量。结果表明，鳙鱼肝脏中至少有3条含汞条带，其等电点分别为3.7、4.8和6.2，草鱼肝脏中有1条含汞条带，等电点为6.2。在鳙鱼等电点为3.7和草鱼等电点为6.2的含汞条带中发现了Se和As。这些条带可能对应于Se对Hg和As毒性的拮抗作用。此外，Hg和As常共存于同一条带中，表明这两种元素可能参与相同的代谢过程。
