Levina E A, Adrianova I Iu, Reunova G D, Zhuravlev Iu N
Genetika. 2008 Mar;44(3):374-80.
The level of within- and among-population variation of larch inhabiting the range of Larix olgensis A. Henry in Primorye was estimated on the basis of 440 RAPD loci identified by means of 12 random primers. In ten population examined, the proportion of polymorphic loci was 35-60%, the average expected heterozygosity varied from 0.1340 to 2169, and the average gene flow estimate was 1.38. According to Fisher's test for heterogeneity, the pairwise differences of the fragment frequencies between the populations were statistically significant. The subdivision index G(ST) = 0.2663 indicated that the interpopulation variation component accounted for approximately 27% of the total variation. Coefficients of Nei's genetic distance between the populations varied from 0.0137 to 0.0934. Most of the samples with high support clustered according to the geographic positions relative to one another within the range. These results suggest that the populations examined are characterized by high genetic variation, like the larch populations of Siberia and the Russian Far East studied earlier, but, in contrast to the latter, exhibit higher among-population variability.
基于用12种随机引物鉴定出的440个RAPD位点,对滨海边疆区红皮云杉分布范围内的红皮云杉种群内和种群间的变异水平进行了评估。在所研究的10个种群中,多态位点比例为35%至60%,平均期望杂合度在0.1340至0.2169之间,平均基因流估计值为1.38。根据费舍尔异质性检验,各群体间片段频率的成对差异具有统计学意义。分化指数G(ST)=0.2663,表明种群间变异成分约占总变异的27%。种群间的奈氏遗传距离系数在0.0137至0.0934之间。大多数具有高支持度的样本根据其在分布范围内相对于彼此的地理位置聚类。这些结果表明,所研究的种群具有较高的遗传变异,与之前研究的西伯利亚和俄罗斯远东地区的落叶松种群类似,但与后者不同的是,它们表现出更高的种群间变异性。