谷胱甘肽与细胞凋亡
Glutathione and apoptosis.
作者信息
Circu Magdalena L, Aw Tak Yee
机构信息
Department of Molecular & Cellular Physiology, Louisiana University Health Sciences Center, Shreveport, Louisiana, USA.
出版信息
Free Radic Res. 2008 Aug;42(8):689-706. doi: 10.1080/10715760802317663.
Abstract
Apoptosis or programmed cell death represents a physiologically conserved mechanism of cell death that is pivotal in normal development and tissue homeostasis in all organisms. As a key modulator of cell functions, the most abundant non-protein thiol, glutathione (GSH), has important roles in cellular defense against oxidant aggression, redox regulation of proteins thiols and maintaining redox homeostasis that is critical for proper function of cellular processes, including apoptosis. Thus, a shift in the cellular GSH-to-GSSG redox balance in favour of the oxidized species, GSSG, constitutes an important signal that could decide the fate of a cell. The current review will focus on three main areas: (1) general description of cellular apoptotic pathways, (2) cellular compartmentation of GSH and the contribution of mitochondrial GSH and redox proteins to apoptotic signalling and (3) role of redox mechanisms in the initiation and execution phases of apoptosis.
摘要
细胞凋亡或程序性细胞死亡是一种生理上保守的细胞死亡机制，在所有生物体的正常发育和组织稳态中起着关键作用。作为细胞功能的关键调节因子，最丰富的非蛋白质硫醇——谷胱甘肽（GSH），在细胞抵御氧化应激、蛋白质硫醇的氧化还原调节以及维持对细胞过程正常功能至关重要的氧化还原稳态（包括细胞凋亡）方面发挥着重要作用。因此，细胞内GSH与GSSG氧化还原平衡向氧化型GSSG的转变构成了一个重要信号，该信号可能决定细胞的命运。本综述将聚焦于三个主要领域：（1）细胞凋亡途径的概述；（2）GSH的细胞区室化以及线粒体GSH和氧化还原蛋白对凋亡信号传导的贡献；（3）氧化还原机制在细胞凋亡起始和执行阶段的作用。
相似文献
1
Glutathione and apoptosis.
Free Radic Res. 2008 Aug;42(8):689-706. doi: 10.1080/10715760802317663.
2
Glutaredoxin 2 catalyzes the reversible oxidation and glutathionylation of mitochondrial membrane thiol proteins: implications for mitochondrial redox regulation and antioxidant DEFENSE.
J Biol Chem. 2004 Nov 12;279(46):47939-51. doi: 10.1074/jbc.M408011200. Epub 2004 Aug 30.
3
Contribution of mitochondrial GSH transport to matrix GSH status and colonic epithelial cell apoptosis.
Free Radic Biol Med. 2008 Mar 1;44(5):768-78. doi: 10.1016/j.freeradbiomed.2007.09.011. Epub 2007 Sep 25.
4
Lipid hydroperoxide-induced apoptosis in human colonic CaCo-2 cells is associated with an early loss of cellular redox balance.
FASEB J. 2000 Aug;14(11):1567-76. doi: 10.1096/fj.14.11.1567.
5
Interaction between glutathione and apoptosis in systemic lupus erythematosus.
Autoimmun Rev. 2013 May;12(7):741-51. doi: 10.1016/j.autrev.2012.12.007. Epub 2012 Dec 29.
6
Redox biology of the intestine.
Free Radic Res. 2011 Nov;45(11-12):1245-66. doi: 10.3109/10715762.2011.611509. Epub 2011 Sep 5.
7
Decreased susceptibility of differentiated PC12 cells to oxidative challenge: relationship to cellular redox and expression of apoptotic protease activator factor-1.
Cell Death Differ. 2005 Aug;12(8):1066-77. doi: 10.1038/sj.cdd.4401650.
8
Apoptosis in mitotic competent undifferentiated cells is induced by cellular redox imbalance independent of reactive oxygen species production.
FASEB J. 2002 Jun;16(8):781-90. doi: 10.1096/fj.01-0784com.
9
Early redox imbalance mediates hydroperoxide-induced apoptosis in mitotic competent undifferentiated PC-12 cells.
Cell Death Differ. 2002 Sep;9(9):1007-16. doi: 10.1038/sj.cdd.4401064.
10
Systemic and strict regulation of the glutathione redox state in mitochondria and cytosol is needed for zebrafish ontogeny.
Biochim Biophys Acta Gen Subj. 2024 Jun;1868(6):130603. doi: 10.1016/j.bbagen.2024.130603. Epub 2024 Mar 21.
引用本文的文献
1
Synergistic targeting of cancer-related proteins by benzyl isothiocyanate and caffeic acid: MLSD and cytotoxic mechanisms in MCF-7 cells.
3 Biotech. 2025 Sep;15(9):291. doi: 10.1007/s13205-025-04469-1. Epub 2025 Aug 11.
2
Realizing highly sensitive electrochemical determination of 2,4,6-trichlorophenol by tailoring the electronic state of a palladium‒carbon cocatalyst.
Mikrochim Acta. 2025 Jun 24;192(7):454. doi: 10.1007/s00604-025-07300-z.
3
Effects of bortezomib on intracellular antioxidant and apoptosis in HepG2cells.
PeerJ. 2025 Apr 28;13:e19235. doi: 10.7717/peerj.19235. eCollection 2025.
4
Metabolomics and transcriptomics analyses reveal the complex molecular mechanisms by which the hypothalamus regulates sexual development in female goats.
BMC Genomics. 2025 Mar 28;26(1):303. doi: 10.1186/s12864-025-11492-2.
5
Glutathione: A Key Regulator of Extracellular Matrix and Cell Death in Intervertebral Disc Degeneration.
Mediators Inflamm. 2024 Oct 1;2024:4482642. doi: 10.1155/2024/4482642. eCollection 2024.
6
Redox-manipulating nanocarriers for anticancer drug delivery: a systematic review.
J Nanobiotechnology. 2024 Sep 28;22(1):587. doi: 10.1186/s12951-024-02859-w.
7
Design and In Vitro Activity of Furcellaran/Chitosan Multilayer Microcapsules for the Delivery of Glutathione and Empty Model Multilayer Microcapsules Based on Polysaccharides.
Materials (Basel). 2024 Apr 26;17(9):2047. doi: 10.3390/ma17092047.
8
In Vitro Low-Bortezomib Doses Induce Apoptosis and Independently Decrease the Activities of Glutathione S-Transferase and Glutathione Peroxidase in Multiple Myeloma, Taking into Account the and Gene Variants.
Genes (Basel). 2024 Mar 21;15(3):387. doi: 10.3390/genes15030387.
9
Arginine with leucine drives reactive oxygen species-mediated integrin α5β1 expression and promotes implantation in mouse blastocysts.
PNAS Nexus. 2024 Mar 12;3(3):pgae114. doi: 10.1093/pnasnexus/pgae114. eCollection 2024 Mar.
10
Sting mediates infection by reducing accumulation of reactive oxygen species.
Infect Immun. 2024 Mar 12;92(3):e0056022. doi: 10.1128/iai.00560-22. Epub 2024 Feb 16.
本文引用的文献
1
Inhibition of caspase-3 activity and activation by protein glutathionylation.
Biochem Pharmacol. 2008 Jun 1;75(11):2234-44. doi: 10.1016/j.bcp.2008.02.026. Epub 2008 Feb 29.
2
Uncoupling protein-2 (UCP2) induces mitochondrial proton leak and increases susceptibility of non-alcoholic steatohepatitis (NASH) liver to ischaemia-reperfusion injury.
Gut. 2008 Jul;57(7):957-65. doi: 10.1136/gut.2007.147496. Epub 2008 Feb 28.
3
Contribution of mitochondrial GSH transport to matrix GSH status and colonic epithelial cell apoptosis.
Free Radic Biol Med. 2008 Mar 1;44(5):768-78. doi: 10.1016/j.freeradbiomed.2007.09.011. Epub 2007 Sep 25.
4
Assessment of endoplasmic reticulum glutathione redox status is confounded by extensive ex vivo oxidation.
Antioxid Redox Signal. 2008 May;10(5):963-72. doi: 10.1089/ars.2007.1869.
5
Nonequilibrium thermodynamics of thiol/disulfide redox systems: a perspective on redox systems biology.
Free Radic Biol Med. 2008 Mar 15;44(6):921-37. doi: 10.1016/j.freeradbiomed.2007.11.008. Epub 2007 Nov 28.
6
The mitochondrial permeability transition regulates cytochrome c release for apoptosis during endoplasmic reticulum stress by remodeling the cristae junction.
J Biol Chem. 2008 Feb 8;283(6):3476-3486. doi: 10.1074/jbc.M707528200. Epub 2007 Dec 5.
7
The hierarchy of structural transitions induced in cytochrome c by anionic phospholipids determines its peroxidase activation and selective peroxidation during apoptosis in cells.
Biochemistry. 2007 Dec 11;46(49):14232-44. doi: 10.1021/bi701237b. Epub 2007 Nov 16.
8
Intracellular GSH levels rather than ROS levels are tightly related to AMA-induced HeLa cell death.
Chem Biol Interact. 2008 Jan 10;171(1):67-78. doi: 10.1016/j.cbi.2007.08.011. Epub 2007 Sep 6.
9
Role of calcium and cyclophilin D in the regulation of mitochondrial permeabilization induced by glutathione depletion.
Biochem Biophys Res Commun. 2007 Nov 23;363(3):572-7. doi: 10.1016/j.bbrc.2007.08.196. Epub 2007 Sep 14.
10
Thioredoxin and TRAF family proteins regulate reactive oxygen species-dependent activation of ASK1 through reciprocal modulation of the N-terminal homophilic interaction of ASK1.
Mol Cell Biol. 2007 Dec;27(23):8152-63. doi: 10.1128/MCB.00227-07. Epub 2007 Aug 27.
Zotero 插件