Roohi Farnoosh, Antonietti Markus, Titirici Maria-Magdalena
Max-Planck Institute for Colloids and Interfaces, Research Campus Golm, Am Mühlenberg 1, D-14476 Golm, Germany.
J Chromatogr A. 2008 Sep 5;1203(2):160-7. doi: 10.1016/j.chroma.2008.07.042. Epub 2008 Jul 19.
One of the recent major improvements of HPLC was the introduction of monolithic silica columns, which have the advantage of faster separation and lower back pressure as compared to common silica beads. Here, we present an interesting alternative to such reversed-phase monolithic columns by a convenient coupling route of a thermo-responsive polymer to hydrophilic silica monoliths. Poly(N-isopropylacrylamide) (PNIPAM) was polymerized in solution via a reversible addition fragmentation chain transfer (RAFT) polymerization technique and coupled then in situ onto an amino-modified silica monolithic column. These columns were compared with RP-18 monolithic columns in the separation of steroids under isocratic condition in aqueous mobile phase. Separation is optimized just by changing the temperature, instead of changing the mobile phase composition.
高效液相色谱法（HPLC）近期的一项重大改进是引入了整体硅胶柱，与普通硅胶珠相比，它具有分离速度更快、背压更低的优点。在此，我们通过一种将热响应聚合物与亲水性硅胶整体柱便捷偶联的途径，提出了一种有趣的替代此类反相整体柱的方法。聚（N-异丙基丙烯酰胺）（PNIPAM）通过可逆加成-断裂链转移（RAFT）聚合技术在溶液中聚合，然后原位偶联到氨基修饰的硅胶整体柱上。在等度条件下，于水性流动相中对这些柱与RP-18整体柱在甾体分离方面进行了比较。仅通过改变温度而非改变流动相组成来优化分离。
