Carvalho-Freitas Maria I R, Rodrigues-Costa Elaine C, Nasello Antonia G, Palermo-Neto João, Felicio Luciano F
Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Neuroimmunomodulation. 2008;15(2):131-9. doi: 10.1159/000148196. Epub 2008 Aug 5.
Prolactin (PRL), a peptide hormone produced by the pituitary gland, is involved in the interaction between the neuroendocrine and immune system. Since dopamine receptor antagonists increase serum levels of PRL, both PRL and dopamine receptors might be involved in the modulation of macrophage activity, providing means of communication between the nervous and immune systems. This study evaluated the effects of PRL and the dopamine antagonist domperidone (DOMP) on macrophage activity of female rats.
Oxidative burst and phagocytosis of peritoneal macrophages were evaluated by flow cytometry. Samples of peritoneal liquid from female rats were first incubated with PRL (10 and 100 nM) for different periods. The same procedure was repeated to evaluate the effects of DOMP (10 and 100 nM).
In vitro incubation of macrophages with 10 nM DOMP decreased oxidative burst, after 30 min, whereas the PMA-induced burst was decreased by DOMP 10 nM after 2 and 4 h. Treatment with PRL (10 and 100 nM) for 30 min decreased oxidative burst and rate of phagocytosis (10 nM). After 2 h of incubation, 10 nM PRL decreased oxidative burst and phagocytosis intensity, but increased the rate of phagocytosis. On the other hand, after 4 h, PRL 10 and 100 nM increased oxidative burst and the rate of phagocytosis, but decreased intensity of phagocytosis.
These observations suggest that macrophage functions are regulated by an endogenous dopaminergic tone. Our data also suggest that both PRL and dopamine exert their action by acting directly on the peritoneal macrophage.
催乳素（PRL）是一种由垂体分泌的肽类激素，参与神经内分泌系统与免疫系统之间的相互作用。由于多巴胺受体拮抗剂会增加血清PRL水平，因此PRL和多巴胺受体可能都参与巨噬细胞活性的调节，从而为神经和免疫系统之间提供沟通方式。本研究评估了PRL和多巴胺拮抗剂多潘立酮（DOMP）对雌性大鼠巨噬细胞活性的影响。
通过流式细胞术评估腹膜巨噬细胞的氧化爆发和吞噬作用。首先将雌性大鼠的腹膜液样本与PRL（10和100 nM）在不同时间段孵育。重复相同步骤以评估DOMP（10和100 nM）的作用。
巨噬细胞与10 nM DOMP体外孵育30分钟后氧化爆发降低，而PMA诱导的爆发在2小时和4小时后被10 nM DOMP降低。用PRL（10和100 nM）处理30分钟可降低氧化爆发和吞噬率（10 nM）。孵育2小时后，10 nM PRL降低氧化爆发和吞噬强度，但增加吞噬率。另一方面，4小时后，10和100 nM PRL增加氧化爆发和吞噬率，但降低吞噬强度。
这些观察结果表明巨噬细胞功能受内源性多巴胺能张力调节。我们的数据还表明，PRL和多巴胺均通过直接作用于腹膜巨噬细胞发挥作用。
