[Sequence analysis of a novel human leukocyte antigen allele HLA-A*9206].

OBJECTIVE

To investigate the molecular genetic basis for a human leukocyte antigen (HLA) novel allele HLA-A*9206 in the Chinese population.

METHODS

DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 1-8 of the proband was preformed and the PCR products were sequenced using ABI sequencing kit. Both strands of exons 2, 3 and 4 of the amplified product were sequenced. The polymerase chain reaction-sequence specific primer (PCR-SSP) was performed to split the two alleles apart and confirm the mutations detected by sequencing.

RESULTS

The sequencing results showed that the HLA-A alleles of the proband were A1101 and a novel allele. The sequence of the novel allele has been submitted to GenBank (EF062306). After Blast analysis, the novel allele shows one nucleotide different from the HLA-A0206 in exon 3 at nucleotide position 530 (C to T). This results in an amino acid change from Ala to Val at codon 153.

CONCLUSION

This allele is a novel allele and has been officially named A*9206 by the WHO Nomenclature Committee.