表皮生长因子可诱导完整细胞中表皮生长因子受体与肌动蛋白之间相互作用的变化。

Epidermal growth factor induces changes of interaction between epidermal growth factor receptor and actin in intact cells.

作者信息

Song Wei, Xuan Haixing, Lin Qishui

机构信息

Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

出版信息

Acta Biochim Biophys Sin (Shanghai). 2008 Aug;40(8):754-60.

PMID:18685792

Abstract

The epidermal growth factor receptor (EGFR) is a cytoskeleton-binding protein. Although purified EGFR can interact with actins in vitro and normally at least 10% of EGFR exist in the insoluble cytoskeleton fraction of A431 cells, interaction of cytosolic EGFR with actin can only be visualized by fluorescence resonance energy transfer when epidermal growth factor presents in the cell medium. Results indicate that the correct orientation between EGFR and actin is important in the signal transduction process.

摘要

表皮生长因子受体（EGFR）是一种细胞骨架结合蛋白。尽管纯化的EGFR在体外能与肌动蛋白相互作用，且在A431细胞的不溶性细胞骨架部分中通常至少有10%的EGFR存在，但只有当细胞培养基中存在表皮生长因子时，胞质EGFR与肌动蛋白的相互作用才能通过荧光共振能量转移观察到。结果表明，EGFR与肌动蛋白之间的正确取向在信号转导过程中很重要。