Bonner Matthew R, Shen Min, Liu Chin-San, Divita Margaret, He Xingzhou, Lan Qing
Department of Social and Preventive Medicine, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY 14214, United States.
Lung Cancer. 2009 Mar;63(3):331-4. doi: 10.1016/j.lungcan.2008.06.012. Epub 2008 Aug 8.
Smoky coal contains polycyclic aromatic hydrocarbons (PAHs) and has been strongly implicated in etiology of lung cancer in Xuan Wei, China. While PAHs form bulky adducts in nuclear DNA, they have a 40-90-fold greater affinity for mitochondrial DNA (mtDNA). mtDNA content may increase to compensate for mtDNA damage. We conducted a population-based case-control study of lung cancer in Xuan Wei, China hypothesizing that mtDNA content is positively associated with lung cancer risk. Cases (n=122) and controls (n=121) were individually matched on age (+/-2 years), sex, village of residence, and current fuel type. Lifetime smoky coal use and potential confounders were determined with questionnaires. mtDNA was extracted from sputum and mtDNA content was determined with quantitative PCR. ORs and 95% CIs were calculated with unconditional logistic regression. mtDNA content >157 copies per cell was associated with lung cancer risk (OR=1.8; 95% CI=1.0-3.2) compared with those with <or=157 copies. In summary, mtDNA content was positively associated with lung cancer risk. Furthermore, mtDNA content was more strongly associated with lung cancer risk among older individuals. However, due to the small sample size, additional studies are needed to evaluate this potential association.
烟煤含有多环芳烃(PAHs),在中国宣威,其与肺癌病因密切相关。虽然PAHs在核DNA中形成大量加合物,但它们对线粒体DNA(mtDNA)的亲和力要高40 - 90倍。mtDNA含量可能会增加以补偿mtDNA损伤。我们在中国宣威开展了一项基于人群的肺癌病例对照研究,假设mtDNA含量与肺癌风险呈正相关。病例(n = 122)和对照(n = 121)按年龄(±2岁)、性别、居住村庄和当前燃料类型进行个体匹配。通过问卷调查确定终生烟煤使用情况和潜在混杂因素。从痰液中提取mtDNA,并通过定量PCR测定mtDNA含量。使用无条件逻辑回归计算比值比(OR)和95%置信区间(CI)。与mtDNA含量≤157拷贝/细胞的人相比,mtDNA含量>157拷贝/细胞与肺癌风险相关(OR = 1.8;95% CI = 1.0 - 3.2)。总之,mtDNA含量与肺癌风险呈正相关。此外,mtDNA含量在老年人中与肺癌风险的关联更强。然而,由于样本量小,需要进一步研究来评估这种潜在关联。