枯草芽孢杆菌对皮肤癣菌毛癣菌属的生物防治

Biological control of dermatophytic fungus Trichophyton species by Bacillus subtilis.

作者信息

Kumar Ajay, Saini Pragati, Shrivastava J N

机构信息

Microbiology Laboratory, Deptt. of Botany, DEI, Deemed University, Dayalbagh, Agra-282005, India.

出版信息

Hindustan Antibiot Bull. 2005;47-48(1-4):36-40.

PMID:18697730

Abstract

A potent Bacillus subtilis-2 isolated from soil samples, showed 14 mm inhibition zone by spot inoculation method and 22 mm inhibition zone by well agar diffusion method against pathogenic test fungii. i.e. Trichophyton species. Among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (TSB) medium at 37 degrees C, pH 7 and 48 hrs of incubation. The MIC of antifungal substance was 35 microl/ml. The isolated Bacillus subtilis -2 has strong antifungal activity against dermatophytic fungi Trichophyton sp. and requires chemical characterization for its bioactive principle.

摘要

从土壤样本中分离出的一种高效枯草芽孢杆菌-2，通过点接种法对致病性测试真菌（即毛癣菌属）显示出14毫米的抑菌圈，通过琼脂扩散法显示出22毫米的抑菌圈。在测试的四种培养基中，在胰蛋白胨大豆肉汤（TSB）培养基中，于37摄氏度、pH值7和培养48小时时发现其生长和抗生素产量最高。抗真菌物质的最低抑菌浓度为35微升/毫升。分离出的枯草芽孢杆菌-2对皮肤癣菌毛癣菌属具有很强的抗真菌活性，其生物活性成分需要进行化学表征。