Electrokinetic behavior of intact wet bone: compartmental model.

Streaming potential experiments were performed on chemically-treated intact wet bone plugs equilibrated in potential-determining ion buffers. Comparison of calculated zeta (zeta) potentials from intact wet bone streaming potentials and bone particle electrophoresis indicates different values. Intact streaming potential experiments, where fluid is forced through the samples, represents flow, primarily through the vascular channel system, and contribution of the organically-lined channels to the electrokinetic zeta potential. Bone particle electrophoresis represents mainly the electrokinetic contribution of exposed mineralized matrix. The organic linings present in the vascular channel system limit potential-determining ions' access to the mineralized matrix. These linings may have an important role in mineral homeostasis and control of ion fluxes between bone compartments.