Bajaj Mini, Gallert Claudia, Winter Josef
Institute of Biology for Engineers and Biotechnology of Wastewater, Am Fasanengarten, University of Karlsruhe, Karlsruhe, Germany.
Chemosphere. 2008 Oct;73(5):705-10. doi: 10.1016/j.chemosphere.2008.06.072.
In this study the continuous treatment of 2-chlorophenol (2-CP) containing synthetic wastewater at increasing concentrations up to 2600 mg L-1 in an anaerobic fixed bed reactor was achieved. As a source of microorganisms municipal sewage sludge was acclimatised to maximally 50 mg L-1 2-CP by 3 successive feedings within 1.5 months. Then, an anaerobic fixed bed reactor was inoculated with this sludge and was operated for 318 d, during which the 2-CP influent concentration was stepwise increased from 50 to 2600 mg L-1 within 265 d. At a hydraulic retention time (HRT) of 2.2 d the 2-CP loading rate was 2 g L-1 d-1 and the average 2-CP removal rate was 0.87 g L-1 d-1, accounting for 73% removal. This is the highest 2-CP removal rate ever reported. The negative effect of a 2-CP loading rate of 1.36 g L-1 d-1 on 2-CP removal was reversible within 2 wk when lower loading conditions (e.g. 0.76 g 2-CP L-1 d-1) were re-established. The median chloride ion release per unit 2-CP degraded was 0.24, which was reasonably close to the theoretically expected value of 0.28. In a batch assay, carried out with relatively clear reactor effluent, the highest removal rate of 2-CP was 175 mg L-1 d-1. At the time of reactor termination on day 318, the 2-CP removal rate by the biofilm in the reactor was 0.61 g L-1 d-1, corresponding to a HRT of 3.4 d and a 2-CP loading rate of 0.76 g L-1 d-1. At these very stable conditions removal of COD was 84% and of 2-CP 81%
在本研究中，在厌氧固定床反应器中实现了对含2-氯苯酚（2-CP）的合成废水进行连续处理，其浓度不断增加，最高可达2600 mg L-1。作为微生物来源，城市污水污泥在1.5个月内通过3次连续进料适应了最高50 mg L-1的2-CP。然后，用该污泥接种厌氧固定床反应器，并运行318天，在此期间，2-CP进水浓度在265天内从50逐步增加到2600 mg L-1。在水力停留时间（HRT）为2.2天的情况下，2-CP负荷率为2 g L-1 d-1，平均2-CP去除率为0.87 g L-1 d-1，去除率为73%。这是迄今为止报道的最高2-CP去除率。当重新建立较低负荷条件（例如0.76 g 2-CP L-1 d-1）时，1.36 g L-1 d-1的2-CP负荷率对2-CP去除的负面影响在2周内是可逆的。每降解单位2-CP释放的氯离子中位数为0.24，这与理论预期值0.28相当接近。在使用相对清澈的反应器流出物进行的批次试验中，2-CP的最高去除率为175 mg L-1 d-1。在第318天反应器终止时，反应器中生物膜对2-CP的去除率为0.61 g L-1 d-1，对应的HRT为3.4天，2-CP负荷率为0.76 g L-1 d-1。在这些非常稳定的条件下，COD的去除率为84%，2-CP的去除率为81%
