Liu Feng, Ying Guang-Guo, Yang Li-Hua, Zhou Qi-Xing
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 511 Kehua Street, Tianhe, Guangzhou, Guangdong Province 510640, China.
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 511 Kehua Street, Tianhe, Guangzhou, Guangdong Province 510640, China.
Ecotoxicol Environ Saf. 2009 Jan;72(1):86-92. doi: 10.1016/j.ecoenv.2008.06.009. Epub 2008 Aug 15.
Triclosan is an antimicrobial agent widely used in many contemporary consumer and health care products. This study assayed phytotoxicity of triclosan using plant growth tests, soil microbial activity using soil respiration and phosphatase activity tests and soil microbial functional diversity using the Biolog ECO plates. The results showed that triclosan inhibited plant growth in soil, with rice seeds being more sensitive than cucumber seeds with EC50 values of 57 and 108 mg/kg. Soil respiration was significantly inhibited in the treatments with triclosan at concentrations more than 10mg/kg (dry soil) during the first 4 days of incubation, but recovered later on after longer incubation. Phosphatase activity was also inhibited for all the soils treated with triclosan (from 0.1 to 50mg/kg dry soil), but a declining inhibition was observed after 2 days of incubation. Biolog analysis found that triclosan treatment increased the utilization of carbon sources and exerted no adverse effects on the functional diversity of soil microbial community.
三氯生是一种广泛应用于许多现代消费品和医疗保健产品中的抗菌剂。本研究采用植物生长试验测定了三氯生的植物毒性，利用土壤呼吸和磷酸酶活性试验测定了土壤微生物活性，并使用Biolog ECO平板测定了土壤微生物功能多样性。结果表明，三氯生抑制土壤中的植物生长，水稻种子比黄瓜种子更敏感，其半数有效浓度（EC50）值分别为57和108mg/kg。在培养的前4天，浓度超过10mg/kg（干土）的三氯生处理显著抑制土壤呼吸，但在更长时间培养后恢复。用三氯生处理的所有土壤（干土浓度为0.1至50mg/kg）的磷酸酶活性也受到抑制，但在培养2天后观察到抑制作用下降。Biolog分析发现，三氯生处理增加了碳源的利用，对土壤微生物群落的功能多样性没有产生不利影响。
